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bsa in pbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bsa in pbs
    Bsa In Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+blocking+buffer/BSA+Blocking+Buffer%2C+5%25+in+PBS/pm42348369-98-18-24
    Average 96 stars, based on 1 article reviews
    bsa in pbs - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: A monoclonal antibody targeting the Nipah virus fusion glycoprotein apex imparts protection from disease.
    Article Snippet: .. Plates were then washed three times with PBS + 0.1% Tween-20 (PBST) and blocked with 100 μL casein in PBS blocking buffer (Thermo Fisher) for 1 hour at room temperature. ..

    Article Title: Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics
    Article Snippet: 50 μL BALF cell suspension or 1 × 10 6 lung cells per well were plated in 96-well microplates (Falcon ® , Corning, USA) and centrifuged (300 g, 5 min, 4 °C). .. Cells were washed with PBS, followed by addition of Fixable Viability Dye (eFluor 780, Thermo Fisher Scientific, USA) for 30 min. After washing with FACS buffer, PBS blocking buffer containing 1% BSA, 5% FCS and 1% CD16/CD32 blocking solution (Thermo Fisher Scientific) was added for 15 min to block a-specific background. .. After another round of washing, the following antibodies were added: Panel 1: BALF cells - CD3e-Pe Cy7, MHCII-FITC, CD11c-APC, B220-Pe Cy7 (Thermo Fisher Scientific) and CCR3-PE (BioLegend, USA) and matching isotypes.

    Article Title: A monoclonal antibody targeting the Nipah virus fusion glycoprotein apex imparts protection from disease.
    Article Snippet: .. Serum samples were serially diluted, in duplicate, in casein in PBS blocking buffer (Thermo Fisher), and 100 μL was added to the wells and allowed to incubate at room temperature for 1 hour. ..

    Article Title: Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics.
    Article Snippet: 50 μL BALF cell suspension or 1 × 106 lung cells per well were plated in 96-well microplates (Falcon®, Corning, USA) and centrifuged (300 g, 5 min, 4 °C). .. Cells were washed with PBS, followed by addition of Fixable Viability Dye (eFluor 780, Thermo Fisher Scientific, USA) for 30 min. After washing with FACS buffer, PBS blocking buffer containing 1% BSA, 5% FCS and 1% CD16/CD32 blocking solution (Thermo Fisher Scientific) was added for 15 min to block a-specific background. .. After another round of washing, the following antibodies were added: Panel 1: BALF cells - CD3e-Pe Cy7, MHCII-FITC, CD11c-APC, B220-Pe Cy7 (Thermo Fisher Scientific) and CCR3-PE (BioLegend, USA) and matching isotypes.

    Article Title: A monoclonal antibody targeting the Nipah virus fusion glycoprotein apex imparts protection from disease.
    Article Snippet: .. Goat anti-hamster IgG (H + L) HRP conjugated secondary antibody (SeraCare 5220-0371) was diluted 1:3,000 in casein in PBS blocking buffer (Thermo Fisher), and 100 μL was added to the wells and allowed to incubate for 1 hour at room temperature. ..

    Article Title: LncR-GAS5 decrease in adenine phosphoribosyltransferase expresssion via binding TAF1 to increase kidney damage created by CIH
    Article Snippet: Cells or tissues were lysed, and the total protein concentration was determined using the BCA detection kit (23227, Thermo Fisher Scientific, USA). .. The proteins were diluted with 5 × loading buffer, the 12 % separation gel was prepared and electrophoresed for 90 min, and proteins were transferred to the membrane for 2 h. Proteins on the membranes were incubated with PBS blocking buffer containing 5 % (w/v) milk powder for 1 h. Next, membranes were incubated overnight at 4 °C with the following primary antibodies: TAF1 (1:300, 50702, Invitrogen, New York, USA), APRT (1:500, ab196558, Cambridge, UK), Bax (1:300, ab32503, Cambridge, UK), Bcl-2 (1:300, ab32124 Cambridge, UK), type I collagen (1:300, ab281974, Cambridge, UK), type IV collagen (1:300, PA5-84585, Invitrogen, New York, USA), LC3-II/LC3-I (1:300, ab192890, Cambridge, UK), p62 (1:300, ab280086, Cambridge, UK), Beclin-1 (1:300, ab210498, Cambridge, UK), and GAPDH (1:500, ab9485, Cambridge, UK). .. After washing away the excess antibodies, the membranes were incubated with a secondary antibody (1:500, ab114610, Abcam, Cambridge, UK) at room temperature for 1 h. Images were captured using a Bio-Rad Imaging System.

    Article Title: Intracellular Protein Binding of Zr-89 Oxine Cell Labeling for PET Cell Tracking Studies
    Article Snippet: .. The PVDF membranes were blocked for non-specific binding with a PBS Blocking Buffer (Thermo Fisher Scientific) and probed with antibodies against histone H3 (clone 3H1), lysine-specific demethylase 1 (LSD1, clone C69G12), or vimentin (clone D21H3) purchased from Cell Signaling Technology for overnight at 4 °C, or with anti-Mek-1 (clone H-8, Santa Cruz Biotechnology, Dallas, TX, USA) or anti-calnexin (polyclonal, Enzo, Farmingdale, NY, USA) antibody for 1 h at room temperature. .. Then the membranes were washed using PBS with 0.1% Tween 20 for 1 h and incubated with a horseradish peroxidase-conjugated anti-rabbit antibody (1:20,000 dilution, Cytiva/GE Healthcare, Marlborough, MA, USA) or anti-mouse IgG (1:1000 dilution, Pierce, Appleton, WI, USA).

    Article Title: Intracellular Protein Binding of Zr-89 Oxine Cell Labeling for PET Cell Tracking Studies
    Article Snippet: .. The PVDF membranes were blocked for non-specific binding with a PBS Blocking Buffer (Thermo Fisher Scientific) and probed with antibodies against histone H3 (clone 3H1), lysine-specific demethylase 1 (LSD1, clone C69G12), or vimentin (clone D21H3) purchased from Cell Signaling Technology for overnight at 4 ◦C, or with anti-Mek-1 (clone H-8, Santa Cruz Biotechnology, Dallas, TX, USA) or anti-calnexin (polyclonal, Enzo, Farmingdale, NY, USA) antibody for 1 h at room temperature. .. Then the membranes were washed using PBS with 0.1% Tween 20 for 1 h and incubated with a horseradish peroxidase-conjugated Pharmaceutics 2025, 17, 518 5 of 17 anti-rabbit antibody (1:20,000 dilution, Cytiva/GE Healthcare, Marlborough, MA, USA) or anti-mouse IgG (1:1000 dilution, Pierce, Appleton, WI, USA).

    FACS:

    Article Title: Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics
    Article Snippet: 50 μL BALF cell suspension or 1 × 10 6 lung cells per well were plated in 96-well microplates (Falcon ® , Corning, USA) and centrifuged (300 g, 5 min, 4 °C). .. Cells were washed with PBS, followed by addition of Fixable Viability Dye (eFluor 780, Thermo Fisher Scientific, USA) for 30 min. After washing with FACS buffer, PBS blocking buffer containing 1% BSA, 5% FCS and 1% CD16/CD32 blocking solution (Thermo Fisher Scientific) was added for 15 min to block a-specific background. .. After another round of washing, the following antibodies were added: Panel 1: BALF cells - CD3e-Pe Cy7, MHCII-FITC, CD11c-APC, B220-Pe Cy7 (Thermo Fisher Scientific) and CCR3-PE (BioLegend, USA) and matching isotypes.

    Article Title: Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics.
    Article Snippet: 50 μL BALF cell suspension or 1 × 106 lung cells per well were plated in 96-well microplates (Falcon®, Corning, USA) and centrifuged (300 g, 5 min, 4 °C). .. Cells were washed with PBS, followed by addition of Fixable Viability Dye (eFluor 780, Thermo Fisher Scientific, USA) for 30 min. After washing with FACS buffer, PBS blocking buffer containing 1% BSA, 5% FCS and 1% CD16/CD32 blocking solution (Thermo Fisher Scientific) was added for 15 min to block a-specific background. .. After another round of washing, the following antibodies were added: Panel 1: BALF cells - CD3e-Pe Cy7, MHCII-FITC, CD11c-APC, B220-Pe Cy7 (Thermo Fisher Scientific) and CCR3-PE (BioLegend, USA) and matching isotypes.

    Membrane:

    Article Title: LncR-GAS5 decrease in adenine phosphoribosyltransferase expresssion via binding TAF1 to increase kidney damage created by CIH
    Article Snippet: Cells or tissues were lysed, and the total protein concentration was determined using the BCA detection kit (23227, Thermo Fisher Scientific, USA). .. The proteins were diluted with 5 × loading buffer, the 12 % separation gel was prepared and electrophoresed for 90 min, and proteins were transferred to the membrane for 2 h. Proteins on the membranes were incubated with PBS blocking buffer containing 5 % (w/v) milk powder for 1 h. Next, membranes were incubated overnight at 4 °C with the following primary antibodies: TAF1 (1:300, 50702, Invitrogen, New York, USA), APRT (1:500, ab196558, Cambridge, UK), Bax (1:300, ab32503, Cambridge, UK), Bcl-2 (1:300, ab32124 Cambridge, UK), type I collagen (1:300, ab281974, Cambridge, UK), type IV collagen (1:300, PA5-84585, Invitrogen, New York, USA), LC3-II/LC3-I (1:300, ab192890, Cambridge, UK), p62 (1:300, ab280086, Cambridge, UK), Beclin-1 (1:300, ab210498, Cambridge, UK), and GAPDH (1:500, ab9485, Cambridge, UK). .. After washing away the excess antibodies, the membranes were incubated with a secondary antibody (1:500, ab114610, Abcam, Cambridge, UK) at room temperature for 1 h. Images were captured using a Bio-Rad Imaging System.

    Incubation:

    Article Title: LncR-GAS5 decrease in adenine phosphoribosyltransferase expresssion via binding TAF1 to increase kidney damage created by CIH
    Article Snippet: Cells or tissues were lysed, and the total protein concentration was determined using the BCA detection kit (23227, Thermo Fisher Scientific, USA). .. The proteins were diluted with 5 × loading buffer, the 12 % separation gel was prepared and electrophoresed for 90 min, and proteins were transferred to the membrane for 2 h. Proteins on the membranes were incubated with PBS blocking buffer containing 5 % (w/v) milk powder for 1 h. Next, membranes were incubated overnight at 4 °C with the following primary antibodies: TAF1 (1:300, 50702, Invitrogen, New York, USA), APRT (1:500, ab196558, Cambridge, UK), Bax (1:300, ab32503, Cambridge, UK), Bcl-2 (1:300, ab32124 Cambridge, UK), type I collagen (1:300, ab281974, Cambridge, UK), type IV collagen (1:300, PA5-84585, Invitrogen, New York, USA), LC3-II/LC3-I (1:300, ab192890, Cambridge, UK), p62 (1:300, ab280086, Cambridge, UK), Beclin-1 (1:300, ab210498, Cambridge, UK), and GAPDH (1:500, ab9485, Cambridge, UK). .. After washing away the excess antibodies, the membranes were incubated with a secondary antibody (1:500, ab114610, Abcam, Cambridge, UK) at room temperature for 1 h. Images were captured using a Bio-Rad Imaging System.

    Binding Assay:

    Article Title: Intracellular Protein Binding of Zr-89 Oxine Cell Labeling for PET Cell Tracking Studies
    Article Snippet: .. The PVDF membranes were blocked for non-specific binding with a PBS Blocking Buffer (Thermo Fisher Scientific) and probed with antibodies against histone H3 (clone 3H1), lysine-specific demethylase 1 (LSD1, clone C69G12), or vimentin (clone D21H3) purchased from Cell Signaling Technology for overnight at 4 °C, or with anti-Mek-1 (clone H-8, Santa Cruz Biotechnology, Dallas, TX, USA) or anti-calnexin (polyclonal, Enzo, Farmingdale, NY, USA) antibody for 1 h at room temperature. .. Then the membranes were washed using PBS with 0.1% Tween 20 for 1 h and incubated with a horseradish peroxidase-conjugated anti-rabbit antibody (1:20,000 dilution, Cytiva/GE Healthcare, Marlborough, MA, USA) or anti-mouse IgG (1:1000 dilution, Pierce, Appleton, WI, USA).

    Article Title: Intracellular Protein Binding of Zr-89 Oxine Cell Labeling for PET Cell Tracking Studies
    Article Snippet: .. The PVDF membranes were blocked for non-specific binding with a PBS Blocking Buffer (Thermo Fisher Scientific) and probed with antibodies against histone H3 (clone 3H1), lysine-specific demethylase 1 (LSD1, clone C69G12), or vimentin (clone D21H3) purchased from Cell Signaling Technology for overnight at 4 ◦C, or with anti-Mek-1 (clone H-8, Santa Cruz Biotechnology, Dallas, TX, USA) or anti-calnexin (polyclonal, Enzo, Farmingdale, NY, USA) antibody for 1 h at room temperature. .. Then the membranes were washed using PBS with 0.1% Tween 20 for 1 h and incubated with a horseradish peroxidase-conjugated Pharmaceutics 2025, 17, 518 5 of 17 anti-rabbit antibody (1:20,000 dilution, Cytiva/GE Healthcare, Marlborough, MA, USA) or anti-mouse IgG (1:1000 dilution, Pierce, Appleton, WI, USA).



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